Analysis of sites of newly integrated DNA in cellular genomes is important to several fields, but methods for analyzing and visualizing these datasets are still under development. Here, we describe tools for data analysis and visualization that take as input integration site data from our INSPIIRED pipeline. Paired-end sequencing allows inference of the numbers of transduced cells as well as the distributions of integration sites in target genomes. We present interactive heatmaps that allow comparison of distributions of integration sites to (...) genomic features and that support numerous user-defined statistical tests. To summarize integration site data from human gene therapy samples, we developed a reproducible report format that catalogs sample population structure, longitudinal dynamics, and integration frequency near cancer-associated genes. We also introduce a novel summary statistic, the UC50, which provides a single number summarizing possible clonal expansion. Using these tools, we characterize ongoing longitudinal characterization of a patient from the first trial to treat severe combined immunodeficiency-X1, showing successful reconstitution for 15 years accompanied by persistence of a cell clone with an integration site near the cancer-associated gene CCND2. Software is available at https://github.com/BushmanLab/INSPIIRED. (shrink)

Dans le contexte des révoltes de Mai 68, certains groupes politiques ont développé des techniques collectives de transformation de soi. On en explore ici deux versions : « l’analyse de groupe », inspirée par la psychanalyse et pratiquée dans le mouvement étudiant ouest-allemand, et le consciousness-raising, utilisé par les féministes radicales étasuniennes. En comparant ces pratiques, on montre que le problème qu’elles posent a concerné un mauvais choix de méthode et non la discussion collective des affects individuels, désormais incriminée par (...) certains récits rétrospectifs sur Mai 68 et critiques libérales. Contre ces dernières, on conclut par une brève défense de la politisation des formes de vie. (shrink)

ABSTRACT The purpose of this study was to develop an understanding of the historical retrospective of the concept of myth and the process of semantic transformation of this term in historical retrospect, as well as to study the features of the combination of different mythological and neo-mythological layers in modern literature. The article investigated myth and mythological discourse in literary studies, formed the basic definitions of myth at different historical stages of social development, studied the foundations of neo-myth and its (...) artistic realization in the literary plane. On the basis of modern Kazakh prose, the plot, composition, motif, and imagery schemes built on the use of mythological and neo-mythological discourses were considered. Emphasis was placed on possible mythological sources and the question of what myth can give to literature was discussed. (shrink)

Divine Creatures: Animal Mummies in Ancient Egypt. Edited by Salima Ikram. Cairo: The American University in Cairo Press, 2015. Pp. xxi + 274, illus. $24.95.

Sous un titre éminemment symbolique, Salima Ghezali, par ailleurs engagée dans la défense des droits de la femme, dresse le portrait d'une famille algérienne éclatée. Des paroles d'hommes et de femmes se succèdent, comme des cris de muezzins dans la nuit, unis par le même amour d'une mère.